With two decades of continuous innovation and customer satisfaction, Meridian Life Science, Inc. has become a recognized leader and preferred partner for the worldwide supply of Goat anti-Human IgG Absorbant.

Problem Interference:

The validity of IgM detection can be compromised by the presence of pathogen-specific (immune) IgG, thereby requiring its removal before testing.  There are two major mechanisms in which immune IgG can interfere with assays for IgM antibodies:

• by competing with specific IgM for substrate binding sites

• by forming immune complexes with rheumatoid factor

Interference by Competitive Binding:

Immune IgG more effectively competes for available binding sites on an antigen substrate than does immune IgM.  In some instances, such as samples with low IgM titers, the competition by immune IgG can effectively block IgM binding and cause a false negative test result.

Interference by Rheumatoid Factor (RF):

Rheumatoid factor (RF) interference presents one of the most serious drawbacks to IgM testing.  RF is an autoimmune antibody (usually an IgM class).  This anti-IgG antibody can bind with immune IgG in the sample to form an immune complex, which can bind to the antigen.  This RF-IgG complex often cannot be distinguished from immune IgM complexes and invariably yields an IgM false positive test result.

Solution:

IgG Absorbant for Improved IgM Detection:  Goat anti-Human IgG (FC), Catalog #L15406G

• Minimizes IgG Antibody Interference

• Minimizes immune IgG complexed with RF